Novel Express qPCR System - ROX Free
The Novel Express qPCR System-ROX Free provides a convenient, simple, rapid, high sensitivity, specificity, stability, and robust set-up for performing quantitative real-time analysis of DNA samples. The kit is with the proprietary concentration with the HotStart DNA polymerase、Novel Green Plus、dNTPs、Mg2+、reaction buffer, and enhance stabilizer. The HotStart DNA polymerase is inactive under the 75°C that avoid the non-specific hybrid or primer dimer at the room temperature. The activation of the reverse transcriptase enzyme is under 95°C for 2 min and the HotStart DNA polymerase will start running, so users can operate the mixture of reaction buffer can under the bench. The Novel Green Plus fluorescent dye was specific binding with the DNA double strands ensure the consistency of the fluorescent signal and PCR Product. Novel Express qPCR System-ROX Free is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer, template solutions, and RNase– free H2O. Reagents are provided with the sufficient amplification reactions of 20 μl each.