Novel Express qPCR System - ROX I
The Novel Express qPCR System-ROX I provides a convenient, simple, rapid, high sensitivity, specificity, stability, and robust set-up for performing quantitative real-time analysis of DNA samples. The kit is with the proprietary concentration with the HotStart DNA polymerase、Novel Green Plus、dNTPs、Mg2+、reaction buffer, enhance stabilizer, and ROX I reference dye. The HotStart DNA polymerase is inactive under the 75°C that avoids the non-specific hybrid or primer dimer at the room temperature. The activation of the reverse transcriptase enzyme is under 95°C for 2 min and the HotStart DNA polymerase will start running, so users can operate the mixture of reaction buffer can under the bench. The Novel Green Plus fluorescent dye was specific binding with the DNA double strands ensure the consistency of the fluorescent signal and PCR Product. The ROX I reference dye is a passive reference, which is used to normalize real-time PCR reactions. ROX dye provides an internal reference to which the reporter dye signal can be normalized, which is necessary to correct for fluorescent fluctuations due to changes in concentration, volume or light source intensity. Normalization of the reporter dye signal results in increased data precision. The Novel Express qPCR System-ROX I is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer, template solutions, ROX I reference Dye (Depending on the use of fluorescent PCR instrument choice), and RNase-free H2O. Reagents are provided with the sufficient amplification reactions of 20 μl each.