HTD™ Nucleolus Stains

  • $420.00


Description

The amphiphilic HTD™ nucleolus targets stains label nucleolus efficiently. The stains are used for labeling the nucleolus of live adherent and suspension cells, as tracers for the dynamics of nucleolus and for multi-generational cellular tracing. HTD™ nucleolus stains have full-color fluorescence characteristics, which emit blue, green, and red fluorescence under UV, blue, and green light excitations, respectively.

Features

  • Full-color fluorescence, HTD™ nucleolus stains have full-color fluorescence characteristics, which emit blue, green, and red fluorescence under UV, blue, and green light excitations, respectively.
  • Application, HTD™ nucleolus stains are applied to nucleolus of live, dead, or fixed cells.
  • Multi-generational tracking, the stains are used for labeling the nucleolus structures of live adherent and suspension cells, as tracers for the dynamics of nucleolus and for multi-generational cellular tracing.
  • Efficiency, incubate the HTD™ Nucleolus stains for 1 minute, then proceed to image cells.
  • Photostability. more than 9 hours in live cells; more than 15 minutes in specimen.
  • Low cytotoxicity, does not affect viability or proliferation.


Performance

  • Multi-generational tracking

HTD™ nucleolus enabled long-term nucleolus tracking in cells. The stain was transferred to daughter cells by dilution of the parent cell, and the staining pattern of HTD™ nucleolus remained in nucleolus for at least six days after staining.

 

  • Efficiency (Staining time)

 

  • Staining in fixed and permeabilized cells

 

  • Photostability – Co-stained with Hoechst

HTD™ nucleolus and Hoechst nucleus were co-stained and irradiated under UV light (λex: 360-370 nm) for 12 minutes. Observed that Hoechst nucleus completely photobleaching only HTD™ nucleolus still photostability.

 

  • Low cytotoxicity
HTD™ nucleolus stains are low cytotoxic and do not perturb normal cell growth, proliferation, and cellular activities, thus serving as powerful tools for the studies in cell biology and cancer cell biology.

Spectural Properties

  • Blue fluorescence channel:Ex/Em: 360-370 / ≥ 420 nm
  • Green fluorescence channel:Ex/Em: 460-490 / ≥ 520 nm
  • Red fluorescence channel:Ex/Em: 525-545 / ≥ 575 nm

 

Protocol Overview


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